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PAX6 Antibody (Center)

PAX6 Antibody (Center)

ArtNr	ABC-AP6929c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PAX6, AN2, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin
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Primary Accession	P26367
Antigen Type	Synthetic Peptide
Application	WB, FC, IF, IHC-P
Bio Background	PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.
Bio References	Firsova, N.V., et.al., Dokl. Biol. Sci. 426, 264-266 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB21476
Gene ID	5080
Gene Name	PAX6
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of PAX6 Antibody (Center)(Cat#AP6929c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	PAX6 Antibody (Center) (Cat.# AP6929c) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Flow cytometric analysis of hela cells using PAX6 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	P55864; P63016; P63015; Q1LZF1
Predicted	B, M, Rat, X
Isotype	Rabbit IgG
Calculated MW	46683
Antigen Region	183-210
Antigen Source	HUMAN
Target/Specificity	This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the Central region of human PAX6.
NCBI Accession	NP_000271.1; NP_001121084.1; NP_001245393.1; NP_001245394.1; NP_001595.2

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Menge: 400 ul

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