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SFRP5 Antibody (Center)

SFRP5 Antibody (Center)

ArtNr	ABC-AP6991C
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SFRP5, FRP1B, SARP3, Secreted frizzled-related protein 5, Frizzled-related protein 1b, Secreted apoptosis-related protein 3, sFRP-5, FRP-1b, SARP-3
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Primary Accession	Q5T4F7
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina.
Bio References	Deloukas, P., et.al., Nature 429 (6990), 375-381 (2004)
Clonality	Polyclonal
Clone/Animal Names	RB21900
Gene ID	6425
Gene Name	SFRP5
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of SFRP5 Antibody (Center) (Cat. #AP6991c) in K562 cell line lysates (35ug/lane). SFRP5 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human hepatocarcinoma with SFRP5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	Flow cytometric analysis of K562 cells using SFRP5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	Q9XSC1
Predicted	B
Isotype	Rabbit Ig
Calculated MW	35563
Antigen Region	219-248
Antigen Source	HUMAN
Target/Specificity	This SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-248 amino acids from the Central region of human SFRP5.
NCBI Accession	NP_003006.2

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