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PKLR Antibody (N-term)

PKLR Antibody (N-term)

ArtNr	ABC-AP7043a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PKLR, PK1, PKL, Pyruvate kinase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase, Pyruvate kinase PKLR, Pyruvate kinase isozymes L/R
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Primary Accession	P30613
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	PKLR is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).
Bio References	Wang, H., et al., Diabetes 51(9):2861-2865 (2002). Valentini, G., et al., J. Biol. Chem. 277(26):23807-23814 (2002). Mateu, E., et al., Hum. Genet. 110(6):532-544 (2002). Indo, Y., et al., Hum. Mutat. 18(4):308-318 (2001). Acan, N.L., et al., J. Enzym. Inhib. 16(5):457-464 (2001).
Clonality	Polyclonal
Clone/Animal Names	RB03692
Gene ID	5313
Gene Name	PKLR
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	The anti-PKLR Pab (Cat. #AP7043a) is used in Western blot to detect PKLR in NIH/3T3 cell lysate.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	PKLR Antibody (S8) (Cat. #AP7043a) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PKLR antibody detected the PKLR protein (arrow).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 3	IHC-P
Dilution image 3	1:50~100
Isotype	Rabbit Ig
Calculated MW	61830
Antigen Region	1-30
Antigen Source	HUMAN
Target/Specificity	This PKLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PKLR.
NCBI Accession	NP_000289.1; NP_870986.1

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Menge: 400 ul

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