CASK Antibody (Center K227)

Synthetic Peptide

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Polyclonal

8573

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of CASK (Center K227) (Cat. #AP7212c) in Hela cell line lysates (35ug/lane).CASK (arrow) was detected using the purified Pab.

1:1000

WB

Anti-CASK (Center K227) Antibody at 1:1000 dilution + SH-SY5Y whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 105 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

M, Rat

105123

HUMAN

NP_001119526.1; NP_001119527.1; NP_003679.2