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ACAT1 Antibody (C-term)

ACAT1 Antibody (C-term)

ArtNr	ABC-AP7560b
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ACAT1, mitochondrial, MAT, ACAT, T2, Acetyl-CoA acetyltransferase, Acetoacetyl-CoA thiolase
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Primary Accession	P24752
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Bio References	Locke, J.A., Prostate 68 (1), 20-33 (2008) Guo, Z.Y., Biochemistry 46 (35), 10063-10071 (2007) Haapalainen, A.M., Biochemistry 46 (14), 4305-4321 (2007)
Clonality	Polyclonal
Clone/Animal Names	RB14644
Gene ID	38
Gene Name	ACAT1
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-ACAT1 Antibody (C-term) at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of anti-ACAT1 Pab (Cat.#AP7560b) in SK-BR-3 cell line lysates (35ug/lane).ACAT1 (arrow) was detected using the purified Pab.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (Cat.#AP7560b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:10~50
Other Accession	Q8HXY6
Predicted	Pr
Isotype	Rabbit Ig
Calculated MW	45200
Antigen Region	296-329
Antigen Source	HUMAN
Target/Specificity	This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1.
NCBI Accession	NP_000010.1

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ABC-AP7560b-datasheet.pdf

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Menge: 400 ul

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