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HSPA1A/HSPA1B Antibody (Y41)

HSPA1A/HSPA1B Antibody (Y41)

ArtNr	ABC-AP7574a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HSPA1A, HSPA1, Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSX70, HSP701/HSP702
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Primary Accession	P08107
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	HSPA1A is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.
Bio References	Mueller, T., et al., Transplantation 78(2):292-295 (2004). Fekete, A., et al., Pediatr. Res. 54(4):452-455 (2003). Broquet, A.H., et al., J. Biol. Chem. 278(24):21601-21606 (2003). Bruce, C.R., et al., Diabetes 52(9):2338-2345 (2003). Anwar, A., et al., J. Biol. Chem. 277(16):14060-14067 (2002).
Clonality	Polyclonal
Clone/Animal Names	RB12700
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	All lanes : Anti-HSPA1A(Y41) Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: C2C12 whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:4000
Legend image 2	Immunohistochemical analysis of AP7574a on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 2	IHC-P
Dilution image 2	1:100
Legend image 3	Immunohistochemical analysis of AP7574a on paraffin-embedded Human colon tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:100
Other Accession	P11147; P63018; P63017; P11142; Q90473; P19378; O73885; P19120; P09446; P48741; Q04967; P17066; P22202; P09435; P14659; P17156; P54652; P34933; Q07439; P02827; P08106; P55063; A5A8V7; P16627; Q4R888; P34931; P0CB32; Q6S4N2; P17879; Q27965; Q61696; Q27975;
Predicted	B, Pig, Pr, E, C, X, Y, Ha, Zf, Hs, D
Isotype	Rabbit Ig
Antigen Region	19-48
Antigen Source	HUMAN
Target/Specificity	This HSPA1A/HSPA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from human HSPA1A/HSPA1B.
NCBI Accession	NP_005336.3; NP_005337.2

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Menge: 400 ul

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