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EphB6 Antibody (N-term S45)

EphB6 Antibody (N-term S45)

ArtNr	ABC-AP7627A
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Ephrin type-B receptor 6, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6, HEP
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Primary Accession	O15197
Antigen Type	Synthetic Peptide
Application	WB, FC, IHC-P
Bio Background	Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.
Bio References	Freywald, A., et al., J. Biol. Chem. 278(12):10150-10156 (2003). Luo, H., et al., J. Clin. Invest. 110(8):1141-1150 (2002). Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). Luo, H., et al., J. Immunol. 167(3):1362-1370 (2001). Tang, X.X., et al., Proc. Natl. Acad. Sci. U.S.A. 97(20):10936-10941 (2000).
Clonality	Polyclonal
Clone/Animal Names	RB1663
Gene ID	2051
Gene Name	EPHB6
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of anti-EphB6 N-term Pab (Cat. #AP7627a) in A549 cell lysate. EphB6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	Flow cytometric analysis of MCF-7 cells using EphB6 Antibody (N-term S45) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	P0C0K7; O08644
Predicted	M, Rat
Isotype	Rabbit Ig
Calculated MW	110700
Antigen Region	45-74
Antigen Source	HUMAN
Target/Specificity	This EphB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human EphB6.
NCBI Accession	NP_001267723.1; NP_001267724.1; NP_004436.3

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