PDGFRA Antibody (Y720)

Synthetic Peptide

PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

Polyclonal

5156

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-PDGFRA Antibody (Y720) at 1:1000 dilution + Mouse brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 123 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

WB

AP7666D staining PDGFRA in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

1:25

IHC-P

P26619; P20786; P26618; Q9PUF6; NP_006197

Rabbit Ig

698-727

This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from human PDGFRA.