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FYN Antibody (N-term)

FYN Antibody (N-term)

ArtNr	ABC-AP7709a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	FYN, Tyrosine-protein kinase Fyn, Proto-oncogene c-Fyn, p59-Fyn, Proto-oncogene Syn, Src-like kinase, SLK
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Primary Accession	P06241
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, WB
Bio Background	FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein.
Bio References	Taniguchi, S., et al., Biochem. Biophys. Res. Commun. 306(1):151-155 (2003). Chan, B., et al., Nat. Cell Biol. 5(2):155-160 (2003). Goldsmith, J.F., et al., Biochem. Biophys. Res. Commun. 298(4):501-504 (2002). Freund, C., et al., EMBO J. 21(22):5985-5995 (2002). Parravicini, V., et al., Nat. Immunol. 3(8):741-748 (2002).
Clonality	Polyclonal
Clone/Animal Names	RB03090
Gene ID	2534
Gene Name	FYN
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Western blot analysis of FYN (arrow) using rabbit polyclonal FYN Antibody (N-term) (Cat. #AP7709a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of anti-FYN (N-term) Pab (Cat. #AP7709a) in mouse liver tissue lysate. FYN (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	FYN Antibody (N-term) (Cat. #AP7709a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	Q62844; P39688; Q05876; A0JNB0
Predicted	B, C, Rat
Isotype	Rabbit Ig
Calculated MW	60762
Antigen Region	13-43
Antigen Source	HUMAN
Target/Specificity	This FYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-43 amino acids from the N-terminal region of human FYN.
NCBI Accession	NP_002028.1; NP_694592.1; NP_694593.1

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