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CASP9 Antibody (S196)

CASP9 Antibody (S196)

ArtNr	ABC-AP7974a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Caspase-9, CASP9, MCH6, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, Caspase-9 subunit p35, Caspase-9 subunit p10, APAF-3, ICE-LAP6, CASP-9
Lieferbar
Primary Accession	P55211
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Bio References	Martin, M.C., et al., J. Biol. Chem. 280(15):15449-15455 (2005). Raina, D., et al., J. Biol. Chem. 280(12):11147-11151 (2005). Cornelis, S., et al., Oncogene 24(9):1552-1562 (2005). Mohammad, R.M., et al., Mol. Cancer Ther. 4(1):13-21 (2005). Tacconi, S., et al., Exp. Neurol. 190(1):254-262 (2004).
Clonality	Polyclonal
Clone/Animal Names	RB6899
Gene ID	842
Gene Name	CASP9
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of anti-CASP9 Antibody (S196) (Cat.#AP7974a) in Jurkat cell line lysates (35ug/lane). CASP9 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human hepatocarcinoma with CASP9 Antibody (S196), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:10~50
Legend image 3	Flow cytometric analysis of MDA-231 cells using CASP9 Antibody (S196)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Isotype	Rabbit Ig
Calculated MW	46281
Antigen Source	HUMAN
Target/Specificity	This CASP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S196 of human CASP9.
NCBI Accession	NP_001220.2; NP_001264983.1; NP_127463.2

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Menge: 400 ul

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