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HLA-B Antibody (N-term)

HLA-B Antibody (N-term)

ArtNr	ABC-AP8616a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HLA-B, HLA class I histocompatibility antigen, HLAB, B-27 alpha chain, MHC class I antigen B*27
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Primary Accession	P03989
Antigen Type	Synthetic Peptide
Application	IHC-P-Leica, FC, WB
Bio Background	HLA-B is involved in the presentation of foreign antigens to the immune system.
Bio References	Higgins, C.M., et.al., Ann. Rheum. Dis. 51 (7), 855-862 (1992) Guild, B.C.et.al., J. Biol. Chem. 259 (14), 9235-9240 (1984) Orr, H.T., et.alt., Biochemistry 18 (25), 5711-5720 (1979)
Clonality	Polyclonal
Clone/Animal Names	RB20998
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-HLA-B Antibody (N-term) at 1:1000-2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Ramos whole cell lysate Lane 5: A375 whole cell lysate Lane 6: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000-2000
Legend image 2	Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using AP8616a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 2	IHC-P-Leica
Dilution image 2	1:500
Legend image 3	Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, Cat#AP8616a) compared to an isotype control of rabbit IgG (blue). AP8616a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Type image 3	FC
Dilution image 3	1:25
Other Accession	P30490; P30488; P30487; P30486; P30485; P30483; P30481; P30479; Q04826; P18463; P30464; P30461
Isotype	Rabbit IgG
Antigen Region	62-90
Antigen Source	HUMAN
Target/Specificity	This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.

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ABC-AP8616a-datasheet.pdf

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Menge: 400 ul

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Lieferung vsl. bis 06.11.2025

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