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MLX Antibody (Center)

MLX Antibody (Center)

ArtNr	ABC-AP8638c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MLX, TCFL4, bHLHd13, Max-like protein X, Max-like bHLHZip protein, Protein BigMax, BHLHD13, Class D basic helix-loop-helix protein 13, Transcription factor-like protein 4
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Primary Accession	Q9UH92
Antigen Type	Synthetic Peptide
Application	WB, FC, IHC-P
Bio Background	MLX belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This protein may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4.
Bio References	Meroni, G., et.al., Oncogene 19 (29), 3266-3277 (2000) Billin, A.N., et.al., J. Biol. Chem. 274 (51), 36344-36350 (1999)
Clonality	Polyclonal
Clone/Animal Names	RB22641
Gene ID	6945
Gene Name	MLX
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of MLX Antibody (Center) (Cat. #AP8638c) in MDA-MB231 cell line lysates (35ug/lane). MLX (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of MLX (arrow) using rabbit polyclonal MLX Antibody (Center) (Cat. #AP8638c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLX gene.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	Flow cytometric analysis of NCI-H292 cells using MLX Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	O08609
Predicted	M
Isotype	Rabbit Ig
Calculated MW	33300
Antigen Region	125-151
Antigen Source	HUMAN
Target/Specificity	This MLX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human MLX.
NCBI Accession	NP_733752.1; NP_937847.1; NP_937848.1

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