CBLB Antibody (Center)

Synthetic Peptide

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. It also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. It may also be involved in EGFR ubiquitination and internalization.

Polyclonal

868

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-CBLB Antibody (Center) at 1:1000 dilution + Rat spleen whole tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 109 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

IHC-P

CBLB Antibody (Center) (Cat. #AP8668c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control-Rabbit
IgG Isotype Control (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

X, M

109450

HUMAN

NP_733762.2