Vergleich

HP Antibody (Center)

ArtNr ABC-AP8929c
Hersteller Abcepta
Menge 400 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products HP, Haptoglobin, Zonulin, Haptoglobin beta chain, Haptoglobin alpha chain
Lieferbar
Primary Accession
P00738
Antigen Type
Synthetic Peptide
Application
FC, WB, IHC-P
Bio Background
HP is a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia.
Bio References
Ryndel, M., et.al., Clin. Chim. Acta 411 (7-8), 500-504 (2010) Igl, W., PLoS Genet. 6 (1), E1000798 (2010)
Clonality
Polyclonal
Clone/Animal Names
RB21463
Gene ID
3240
Gene Name
HP
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M
Legend image 1
Western blot analysis of HP (arrow) using rabbit polyclonal HP Antibody (Center) (Cat. #AP8929c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HP gene (Lane 2) .
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
Anti-HP Antibody (Center) at 1:8000 dilution + Human liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 45, 38 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:8000
Legend image 3
AP8929c staining HP in human epatocarcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 3
IHC-P
Dilution image 3
1:25
Legend image 4
HP Antibody (Center) (Cat. #AP8929c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4
FC
Dilution image 4
1:10~50
Isotype
Rabbit Ig
Calculated MW
45205
Antigen Region
295-322
Antigen Source
HUMAN
Target/Specificity
This HP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-322 amino acids from the Central region of human HP.
NCBI Accession
NP_001119574.1; NP_005134.1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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