THP-1 Cell Slide

THP-1 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.

Human THP-1 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. The THP-1 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 THP-1 cells. A follow-up heat dehydration process was done to ensure the attachment of THP-1 cells and the stability of cellular proteins.

THP-1

5 slides/pack

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