Chicken polyclonal antibody to human GFRa-2

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GFRa-2 is a member of the GDNF receptor family. It is a glycosylphosphatidyl-inositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NRTN compared to its other family member, GDNF family receptor alpha 1. http://www.ncbi.nlm.nih.gov/gene/2675

Chicken polyclonal

Recombinant His-tagged human GFRalpha-2 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRalpha-2, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant

WB 0.2 to 1 ug/ml; ELISA: 6.25 to 8 ng/ml; IF 1 to 2 ug/ml
Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

-

O00451