Mouse monoclonal antibody to HIV-1 Tat

Mouse monoclonal

Recombinant Tat protein of HIV-1 BH10 (purified from E. coli)

ELISA 0.16 to 0.8 ug/ml; WB 2 ug/ml

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Campioni, D., Corallini, A., Zauli, G., Possati, L., Altavilla, G. and Barbanti-Brodano, G (1995). HIV type I extracellular Tat protein stimulates growth and protects cells of BK virus/tat transgenic mice from apoptosis. AIDS Res Hum Retroviruses 11, 1039-1048. PMID: 8554901

Huang L, Bosch I, Hofmann W, Sodroski J, Pardee AB. (1998). Tat protein induces human immunodeficiency virus type 1 (HIV-1) coreceptors and promotes infection with both macrophage-tropic and T-lymphotropic HIV-1 strains. J Virol. 72(11):8952-60. PMID: 9765440

Rohr O, C Schwartz, C Hery, D Aunis, M Tardieu and E Schaeffer (2000). The nuclear receptor COUP-TF interacts with HIV-1 Tat and stimulates viral replication in human microglial cells. Journal of Biological Chemistry. 28; 275(4): 2654-60. PMID: 10644726

Arp J, Rieder MJ, Urquhart B, Freeman D, Tucker MJ, Krizova A, Lehmann D, Dekaban GA (2005). Hypersensitivity of HIV-1 infected cells to reactive sulfonamide metabolites correlated to expression of the HIV-1 viral protein tat. J. Pharmcol Exp Ther 314:1218-25. PMID: 15933158

Karasev AV, Foulke S, Wellens C, Rich A, Shon KJ, Zwierzynski I, Hone D, Koprowski H, Reitz M. (2005). Plant based HIV-1 vaccine candidate: Tat protein produced in spinach. Vaccine. 7; 23(15):1875-80. PMID: 15734059

Abbott KL, Archambault J, Xiao H, Nguyen BD, Roeder RG, Greenblatt J, Omichinski JG, Legault P. (2005) Interactions of the HIV-1 Tat and RAP74 Proteins with the RNA Polymerase II CTD Phosphatase FCP1. Biochemistry 44, 2716-2731. PMID: 15723517
Rolland M, Jensen MA, Nickle DC, Yan J, Learn GH, 1 Heath L, Weiner D, Mullins JI.(2007) Reconstruction and Function of Ancestral Center-of-Tree Human Immunodeficiency Virus Type 1 Proteins. Journal of Virology, Vol. 81, No. 16