Blasticidin S HCl, 1 G

X1339381-2

Blasticidin S is an aminoacylnucleoside antibiotic isolated from Streptomyces griseochromogenes . It shows high efficacy against both eukaryotic and prokaryotic cells. Aside from its antimicrobial properties, blasticidin has displayed antiviral, antitumor, and antifungal properties and has been used as a selection agent. It has been used to select for cells containing the bls , bsr , or BSD resistance gene as well as HEK293-T cells and HEK-D5 cells. Additionally, blasticidin is effective in preventing cell culture contamination and studying protein synthesis. Blasticidin S functions by inhibiting peptide bond formation at the ribosome. It blocks hydrolysis of peptidyl-tRNA due to the large ribosomal subunit's increased affinity for tRNA. Blasticidin S, longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system 1, 2 . Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in Duchenne muscular dystrophy (DMD) 3 . 1.) http://www.genetics.org/content/genetics/197/4/1069.full.pdf 2.) http://www.g3journal.org/content/early/2016/04/11/g3.116.028241.full.pdf+html 3.)http://nar.oxfordjournals.org/content/44/3/1449.full.pdf+html

218.0-225.0 °C

772.1 °C at 760 mmHg