PurKine His Tagged Protein Purification Protein Purification Kit

- High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin.
-Versatile—For purification under native or denaturing conditions.
- Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
- Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
- Easy to use— Pre-formulated buffers available for kit formats.

PurKine His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine His-Tag Purification resin is also available as prepacked spin column and kit formats.