Human TGF-β1 Pre-Coated ELISA Kit

The three mammalian isoforms of TGF-β , TGF-β 1, β 2, and β 3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-β 1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors.

The BioGems Human TGF-β 1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TGF-β 1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TGF-β 1. It contains CHO-expressed recombinant Human TGF-β 1. The detection antibody is a biotinylated goat polyclonal antibody specific for TGF-β 1. The kit is analytically validated with ready to use reagents.