Alzheimers Disease B-Amyloid Protein Detection Set

These polyclonal antibodies can be used for detection of APP, BACE and BACE2 by immunoblot at 1 - 5 μgg/mL. APP and BACE antibodies can detect their respective proteins via immunohistochemistry at 2 - 10 μg/mL.

Accumulation of the amyloid-β peptide (Aβ ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The β amyloid protein precursor (APP) is cleaved by one of two β secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for β secretase to generate the 4 kDa amyloid-β peptide (Aβ ), which is deposited in the Alzheimer’s disease patients’ brains. BACE was identified by several groups independently and designated β -site APP cleaving enzyme (BACE) . BACE is a transmembrane aspartic protease and co-localizes with APP. BACE2 also cleaves APP at β -site and at a different site within Aβ . BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region’, suggesting that BACE2 and Aβ may also contribute to the pathogenesis of Down syndrome.

For images please see PDF data sheet

Antibodies are supplied as affinity chromatography purified IgG.

PBS containing 0.02% sodium azide.

Polyclonal

Optimal dilutions for each application to be determined by the researcher.