pEASY®-Blunt El Expression Kit

pEASY®-Blunt El Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.


•5 minutes fast ligation of P/u-amplified PCR products.
• Ampicillin resistance.
•T7 promoter primer and T7 terminator primer for sequencing.
•Bacteriophage T7 lac promoter for high level expression.
•N-terminal 6xHis tag for easy purification.
•Transl-Tl phage resistant chemically competent cell, high transformation efficiency(> 109 cfu/µg pUC19 DNA) and fast growmg.
•El Expression Plasmid included as negative control.


Storage
Trans 1-Tl Phage Resistant Chemically Competent Cell at -70℃ for six months; others at -20℃ for nine months


Shipping
Dry ice (-70℃)