KRISHZYME™ Hyaluronidase Enzymatic Assay Kit

2 - 8°C

Assay for estimation of Hyaluronidase activity (Hase activity) in cell culture supernatant and other pharmaceutical samples

Introduction:
Hyaluronidase is a family of enzymes that degrade hyaluronic acid by catalyzing the hydrolysis of hyaluronan, a
constituent of the interstitial barrier. Hyaluronidase lowers the viscosity of hyaluronan, thereby increasing tissue
permeability. It is, therefore, used in medicine in conjunction with other drugs to speed their dispersion and
delivery.

Intended Use:
The KRISHZYME™ Hyaluronidase Assay kit is intended for determination of Hyaluronidase enzyme activity in
cell culture supernatant and pharmaceutical preparations.

Principle:
The KRISHZYME™ Hyaluronidase Assay kit uses a two-step turbidimetric reaction to measure hyaluronidase
activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms
turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at 600 nm is directly proportional to
hyaluronidase activity in the sample.