KRIBIOLISA™ E.coli Host Cell DNA Kit

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E.coli Host Cell DNA Kit

Introduction:
For the production of biotechnological products, expression of therapeutic proteins in E.coli cells is commonly
used. Due to which there is a possibility of DNA contamination from the host cells in the products which are
manufactured. However, the limit of DNA contamination from the cell lines has been set by regulatory agencies.
According to WHO the limit of DNA contamination should not exceed 10 ng/dose in the products manufactured.
One of the most common method which is widely used for detection of host cell DNA contamination by
biopharmaceutical manufacturers is the PicoGreenP
TMP Dye based assay. The research and development
department at Krishgen Biosystems has designed a kit to detect and quantify host cell DNA in the products
manufactured by recombinant expression in E.coli cell lines based on PicoGreenP
TMP Dye assay.

Principle:
The Krishgen E.coli Host Cell DNA Kit is based on the DNA dye binding assay which utilizes PicoGreenP
TM Pdye.
The DNA samples and the standards are reacted with PicoGreenP
TMP dye, which is a DNA intercalator that binds
strongly to the double stranded DNA. Upon binding to DNA, PicoGreenP
TMP dye fluoresces with an excitation of 485
nm and emission of 525 nm. The intensity of the fluorescent signal is proportional to the quantity of DNA in the
standard or the samples.