KRIBIOLISA™ CHO Host Cell DNA Kit

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CHO Host Cell DNA Kit

Background
For the production of biotechnological products, expression of therapeutic proteins in CHO cells is
commonly used. Due to which there is a possibility of DNA contamination from the host cells in the
products which are manufactured. However, the limit of DNA contamination from the cell lines has
been set by regulatory agencies According to WHO the limit of DNA contamination should not
exceed 10ng/dose in the products manufactured. One of the most common method which is
widely used for detection of host cell DNA contamination by biopharmaceutical manufacturers is
the PicoGreenTM Dye based assay. The research and development department at Krishgen
Biosystems has designed a kit to detect and quantify host cell DNA in the products manufactured
by recombinant expression in CHO cell lines based on PicoGreenTM Dye assay.

Principle
The Krishgen CHO Host Cell DNA Kit is based on the DNA dye binding assay which utilizes
PicoGreenTM dye. The DNA samples and the standards are reacted with PicoGreenTM
dye, which is a
DNA interculator that binds strongly to the double stranded DNA. Upon binding to DNA, PicoGreenTM
dye fluoresces with an excitation of 485 nm and emission of 525 nm. The intensity of the fluorescent
signal is proportional to the quantity of DNA in the standard or the samples.