KRIBIOLISA™ Teriparatide (PTH -34) ELISA

2 - 8°C

Immunoassay for quantitative estimation of Teriparatide in human serum, plasma and tissue extracts

Introduction:
Teriparatide is a recombinant protein form of parathyroid hormone consisting of the first (N-terminus) 34 amino
acids, which is the bioactive portion of the hormone. It is an effective anabolic (i.e., bone growing) agent used
in the treatment of some forms of osteoporosis. The Teriparatide ELISA kit is designed to measure
teriparatide with high specificity while minimizing crossreactivity to human PTH (1-84) and other PTHrPs.

Intended Use:
The Teriparatide ELISA is used for estimation of Teriparatide in serum, plasma, or tissue extracts in
pharmacokinetics, peptide delivery study and other purposes.

Principle:
The Teriparatide ELISA is a competitive immunoassay for the determination of Teriparatide. The antiserum is
captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying
concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured
Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which
produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing
an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to
yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific
Teriparatide bound.