Anti-Human IgG, Pan [Clone HP6017] - HRP - 500 µg

Human Immunoglobulin, IgG Pan

Humans produce five classes of immunoglobulins, IgM, IgD, IgA, IgE, and IgG1. IgG is themost abundant class1 and functions in the humoral immune response2. There are four subclassesof IgG, and they are numbered according to decreasing abundance as IgG1 through IgG4. Thefour subclasses are highly conserved and consist of four polypeptide chains: two identical heavychains and two identical light chains1. While the general structure is very similar between IgGsubclasses, differences in their constant regions in the hinge and upper CH2 domain as well as inglycosylation affect their binding to antigens (e.g. pathogenic proteins, polysaccharides, allergens), C1q, and Fc receptors, leading to differences in functionality. As a result, each IgGsubclass has a unique response skewed to specific antigens. IgG1 is the most abundant subclass of IgG and is primarily induced by soluble protein antigensand membrane proteins1. IgG2 responds to bacterial capsular polysaccharide antigens. IgG3 is apro-inflammatory antibody that potently induces effector function. IgG4 is induced by repeatedor chronic exposure to antigens that are non-infectious, such as allergens or antigens releasedduring parasitic worm (helminth) infection. HP6017 was generated by immunizing a BALB/c mouse with purified human immunoglobulins3. Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

0.5 mg/ml

IgG is produced by B cells and is present in mucosal secretions and serum.