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Mouse Pancreas Tissue Lysate

ArtNr LEIN-P187-0.1mg
Hersteller Leinco Technologies
Menge 0.1 mg
Kategorie
Typ LYSATE
Specific against other
Host Human
ECLASS 10.1 32190102
ECLASS 11.0 32190102
UNSPSC 41105330
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Applications
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
Manufacturer - Category
Cell and Tissue Lysates>Mouse Tissue
Shipping Temperature
Next Day 2-8°C
Storage Conditions
This lysate should be aliquoted in working amounts and may be stored for up to one month at 2-8˚C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Expiration Date
One half year from date of receipt
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Product Preparation
Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

Safety Testing

This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.

Tissue Type
Pancreas
Diagnosis
Normal
Safety Testing
This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.1 mg
Lieferbar: In stock
lieferbar

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