RI(dl)-2 (TFA)

435.59

10 mM in DMSO

Cell Cycle/DNA Damage

RI(dl)-2 TFA is a potent and selective RAD51-mediated D-loop formation inhibitor with an IC₅₀ of 11.1 uM. RI(dl)-2 TFA does not influence RAD51 binding to ssDNA and inhibits homologous recombination (HR) activity in human cells (IC₅₀ of 3.0 uM)^[1]. IC50 & Target: IC50: 11.1 uM (RAD51-mediated D-loop formation)^[1] In Vitro: RI(dl)-2 stabilizes nucleoprotein filaments in a nonfunctional state, which are incapable of D-loop activity and simultaneously shielded from related (e.g., RAD52-mediated) pathways that promote single-strand annealing (SSA)^[1].
RI(dl)-2 does not modulate the affinity of RAD51 to ssDNA or the stability of preformed RAD51-ssDNA complexes when challenged with high concentrations of salt^[1].