Camptothecin

7689-03-4

348.35

DMSO : 6.25 mg/mL (17.94 mM; Need ultrasonic); H2O : < 0.1 mg/mL (insoluble)

Anti-infection; Apoptosis; Cell Cycle/DNA Damage; Antibody-drug Conjugate/ADC Related; Anti-infection; Anti-infection

Camptothecin (Campathecin) is a potent DNA enzyme topoisomerase I inhibitor, with an IC50 of 679 nM.
IC50 & Target: IC50: 679 nM (topoisomerase I)
In Vitro: [3H]BrCPT labeling of topoisomerase I is enhanced greatly by the presence of DNA; very little labeling of isolated topoisomerase I or isolated DNA occurrs. Even in the presence of DNA, [3H]BrCPT labeling of topoisomerase I is inhibited by camptothecin, suggesting that both CPT and BrCPT bind to the same site on the DNA-topoisomerase I binary complex. With increasing concentrations of camptothecin, closed circular pRR322 DNA (form I) is converted to nicked circular DNA (form 11). This apparent nicking activitv of camptothecin required DNA topoisomerase I.