DFHBI-1T

Description: DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore used for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers.Exhibits peak excitation maxima of 482 nm and peak fluorescence emissionof 505 nm when bound to Spinach2, enabling imaging with GFP filtercubes. Exhibits higher specific fluorescence and lower backgroundfluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

References: Song W, Strack RL, Svensen N, Jaffrey SR. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29; 136(4):1198-201. doi: 10.1021/ja410819x. Epub 2014 Jan 18. PubMed PMID: 24393009; PubMed Central PMCID: PMC3929357.