Hersteller |
NKMAX
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Applikationen |
SDS-PAGE |
Menge |
500ug |
ArtNr |
NKM-ATGP3913-500ug |
Konjugat/Tag |
His-Tag |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Activity |
Specific activity is > 9, 000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C. |
Antigen Species |
Mouse |
Applications info |
SDS-PAGE, Enzyme Activity |
Biological activity |
Specific activity is > 9, 000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C. |
Concentration |
0.25mg/ml (determined by Absorbance at 280nm) |
Description |
Granzyme B, also known as Gzmb, is a member of the granzyme family of cell death-inducing serine proteases specially expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. It plays crucial role for target cell lysis in cell-mediated immune responses. This protein is linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. It cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Recombinant mouse Granzyme B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
Domain |
19-247aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
Baculovirus |
Formulation |
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Gene Category |
Apoptosis, Enzymes & Proteases |
General References |
D'Eliseo D., et al. (2016) J Exp Clin Cancer Res. 35:24. Rabani M., et al. (2018) Clin Immunol. 188:45-51. |
Molecular Weight |
26.3kDa (235aa) |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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