Human TGF beta 1 ELISA Kit

Transforming growth factor beta 1 or TGF-beta1, encoded by TGFB1 gene in humans, is a secreted protein belonging to the TGF superfamily. TGF-beta1 is synthesized as a 390 amino acid (aa) prepropeptide composed of a 23 aa signal peptide, a 255 aa pro-region, and a 112 aa mature segment. Post-translation of TGF-beta1 includes removal of the signal peptide and glycosylation of the pro-region. The glycosylation process contains the unusual attachment of mannose-6 phosphate residues and furin convertase-mediated cleavage of the prohormone, creating an 80 kDa disulfide-linked pro-region (termed LAP for latency-associated protein), and a 25 kDa disulfide-linked mature segment (termed TGF-beta1). Interaction of these two peptides keeps TGF-beta1 in an inactive state. To facilitate secretion and extracellular storage, a third 200 kDa component termed LTBP is covalently-linked to the N-terminus of one of the two LAP polypeptide chains. Mature human TGF-beta1 is 99% identical to mouse counterpart. The TGF-beta1 receptor is a heterodimeric complex composed of a constitutively phosphorylated, ligand-binding glycoprotein (TbetaRII) and a signal-transducing, non-ligand-binding glycoprotein (TbetaRI/ALK-5). Data available suggested that TGF-beta1 first binds TbetaRII which then phosphorylates TbetaRI and activates the downstream signaling pathways. In addition, a third TGF-beta receptor may also be involved in TGF-beta1 mediated cellular processes. TGF-beta1 plays an important role in immunoregulation, cell proliferation, differentiation and survival. For example, it regulates dendritic cell chemotaxis by altering the expression of chemokine receptors. It can inhibit inflammatory response by dampening macrophage activity and proinflammatory secretion.

1.0 pg/mL

Store at 2 - 8C. Keep reconstituted standard and detection Ab at -20 C

Chromogenic

ELISA Kits