Human TLR2 ELISA Kit

Toll-Like Receptor 2 (TLR2), also known as Toll/interleukin-1 receptor-like protein 4 or CD282, is a transmembrane protein belonging to the TLR family. Mature human TLR2 consists of a 570 amino acid (aa) extracellular domain (ECD) with 19 leucine rich repeats (LLR) and one C-terminal leucine-rich domain, a 21 aa transmembrane segment, and a 175 aa cytoplasmic domain. ECD of human TLR2 shares 67% aa sequence identity with mouse and rat TLR2. TLR2 is expressed on antigen presenting cells (plasmacytoid dendritic cells, B cells, monocytes, and macrophages), T cells (activated CD4+, CD8+, memory, and Treg), renal tubule epithelial cells, trophoblasts, and decidual and amnion epithelial cells. The soluble forms of TLR2 function as decoy receptors that suppress TLR2 mediated inflammation but are unable to bind ligand. TLR2 elicits its effects by interacting with multiple proteins in the plasma membrane including TLR1, TLR6, TLR10, CD14, CD36, CXCR4, Dectin-1, and Integrins I+/-Vbeta3 and I+/-3beta1, as well as the ganglioside GD1a. TLR2-containing receptor complexes are differentially responsive to microbial lipopeptides, peptidoglycans, lipoteichoic acid, enterotoxin, LPS, as well as yeast and parasite glucans. TLR2 is also activated by endogenous ligands such as amyloid Abeta peptide oligomers, Serum Amyloid A, HMGB1, beta-Defensin 3, PAUF, Biglycan, and LMW Hyaluronan. Ligand binding by TLR2 can induce receptor association with CXCR4, resulting in inhibition of CXCR4 and TLR2 signaling. TLR2 initiates proinflammatory signaling through its cytoplasmic TIR domain. TLR2 signaling also regulates adaptive immune responses such as dendritic cell activation, B cell maturation, and T cell mediated tumor regression.

156-10, 000pg/mL

100 uL final volume, dilution factor varies on samples.

Human ELISA Kits