Interleukin-13 Variant Human Recombinant ( IL 13 Variant Human )

Interleukin-13 Variant Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13, having a molecular mass of 12.5 kDa. The IL-13 Variant is purified by proprietary chromatographic techniques.

Greater than 95% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.

Sterile Filtered White lyophilized (freeze-dried) powder.

The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be 1, 000, 000 units/mg. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13.

Immunology