BacMam NT H8-CL7-eGFP

BacMam NT His8-CL7-eGFP enables target proteins to be labeled with N-terminal His8, CL7, and eGFP tags. Protease cleavage removes all tags.EXPRESSION: The plasmid is designed for transient expression in mammalian cells or for baculovirus transduction of mammalian cells (BacMam). Expression is constitutive and driven by the CMV promoter; a Kozak sequence is immediately upstream of the start codon. PEPTIDE TAG:The N-terminal CL7 tag is between the His8 and eGFP tags, and a PreScission protease (PSC) cleavage site is C-terminal to all tags.CLEAVAGE SITE(S): C-terminal PSC P OTHER TAGS: His8 tag is N-terminal, followed by CL7, then eGFP. VOLUME | 10 ug at 500 ng/uL; EXPIRATION | 12 months from receipt when stored as directed. Plasmid guide is available at TriAltusbioscience.com