Oligo, Random Primer 8, Biotinylated (5’-Biotinylated Octamer)

Molecular Biology / MB-Cloning-Primers

-20°C

Supplied as a lyophilized powder. Conjugated at the 5’ end to Biotin.

Single-stranded oligonucleotide primer, chemically synthesized, consisting of 8 random sequence deoxyribonucleotides conjugated to Biotin; suitable for DNA synthesis applications using Klenow DNA polymerase with a template or for cDNA synthesis using reverse transcriptase with an mRNA template.

Sequence:
5’ [Biotin-NNN NNN NN] 3’
[N = A, C, G, T]

Molecular Weight:
2878.1g/mol

GC Content:
~50%

Tm:
24°C

Storage & Stability:
Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.