Oligo, Restriction Site, Nsi I (12mer)

Molecular Biology / MB-Cloning-Restriction Site Primers

-20°C

Supplied as a salt-free lyophilized powder. Reconstitute with sterile dH2O, TE or Tris buffer, pH 7.0.

Single-stranded oligonucleotide, chemically synthesized, suitable for DNA applications.

Special Labels: Biotin, Fluorescein, and Phosphate modifications can be included at the 5' or 3' site. For special labels add -B, -F, -P and -5, -3 to the catalog number.

Sequence:
5' TGC ATG CAT GCA 3'

Molecular Weight:
3645.4g/mol

GC Content:
50%

Tm:
36°C

Synthesis Scale:
50nmole
Synthesis scale refers to the amount of the 3'-terminus base that the multi-step synthesis starts with. Final yield is indicated on the lot-specific certificate of analysis.

Minimum Guaranteed Yield:
1.5 OD (A260nm) unit per mL reconstituted product
1 OD unit corresponds to ~33ug ssDNA

Storage & Stability:
Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.