Rabbit antibody to GAPDH

FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed.
CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.

IHC, WB. A concentration of 1-5 ug/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Fort P, et al. Nucleic Acids Res. 13:1431-1442(1985).

A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

http://ca.expasy.org/uniprot/P04797
http://ca.expasy.org/uniprot/P16858
http://ca.expasy.org/uniprot/P04406

Polyclonal

Lyophilised