Rabbit antibody to KCNJ1

FUNCTION: In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.
Tissue specificity: In the kidney and pancreatic islets. Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Mainly in kidney (renal cortex, medulla and papilla). In the brain, found in the cortex and the hippocampus.
Defects in KCNJ1 are the cause of Bartter syndrome type 2 (BS2) also termed hyperprostanglandin E syndrome 2. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS2 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS2 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.

IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Ho K, et al. Nature 362:31-38(1993)
2. Beesley A.H, et al. Am. J. Physiol. 276:C585-C592(1999)

A synthetic peptide from c-terminal region of mouse KCNJ1 (ROMK1, Kir1.1) conjugated to an immunogenic carrier protein was used as the antigen.

http://www.uniprot.org/uniprot/P48048
http://www.uniprot.org/uniprot/P35560
http://www.uniprot.org/uniprot/O88335

Polyclonal

Lyophilised