Rabbit to antibody to Runx1 (200-260)

FUNCTION: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
SUBUNIT: Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with MYST3 and MYST4. Interacts with SUV39H1, leading to abrogate the transactivating and DNA-binding properties of RUNX1.
TISSUE SPECIFICITY: Highest levels in thymus, bone marrow and peripheral blood.

IHC, WB. A concentration of 10-50 ug/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Miyoshi H, et al. Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
2. Tahirov T.H, et al. Cell 104:755-767(2001).

A synthetic peptide from aa region 200-260 of human Runx1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

http://kr.expasy.org/uniprot/Q01196
http://kr.expasy.org/uniprot/Q63046
http://kr.expasy.org/uniprot/Q03347

Polyclonal

Lyophilised