Rabbit antibody to VGluT2

FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
TISSUE SPECIFICITY: Expressed in brain. Expressed in hippocampal neurons (at protein level).
DEVELOPMENTAL STAGE: Expressed in brain throughout development. Transiently expressed in hippocampal neurons during the first week after birth, with expression decreasing thereafter.
MISCELLANEOUS: Mice lacking this protein exhibit an elevated rate of perinatal lethality. Surviving animals display a strong reduction in evoked glutamergic responses in thalamic neurons. Reduction of protein level in homozygous and heterozygous knockouts leads to a graded reduction in the amplitude of the postsynaptic response to single vesicle fusion in thalamic neurons, consistent with a role for this protein in determining quantal size.

IHC, WB. A dilution of 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Li JL, et al. J Comp Neurol 457:236-49 (2003).

A synthetic peptide from mouse VGluT2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.

http://ca.expasy.org/uniprot/Q9JI12
http://ca.expasy.org/uniprot/Q8BLE7

Polyclonal

Lyophilised