CheKine™ Micro Total Iron-Binding Capacity (TIBC) Assay Kit

CheKine™ Micro Total Iron-Binding Capacity (TIBC ) Assay Kit provides a simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples. The principle is that under alkaline conditions, serum transferrin can be bonded to Fe3+, and the remaining unbound Fe3+ can be reduced to Fe2+, the bonded Fe3+ could be released after acidification , and then be reduced into Fe2+ . A purple compound could be produced by the reaction between Fe2+ and philo oxazine , and this purple compound has a special wave at 562 nm. Respectively reading the OD562nm values A1 and A2 , and A1 is based on the unbound Fe3+ , A2 is based on the bounded Fe3+ . And the concentration of TIBC is proportionate to the difference between A2 and A1.

• simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples .
• Provides detailed sample preparation and results calculation methods.

The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.

Total Iron-Binding Capacity (TIBC ) refers to the ability of serum transferrin to combine iron, and its content is closely related to the occurrence of iron deficiency anemia, acute hepatitis and other diseases.

Total Iron Binding Capacity ，TIBC