ReverTra Ace qPCR RT Master Mix with gDNA Remover

cDNA synthesis for real-time PCR

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This kit includes master mix reagents for reverse transcription and for the removal of genomic DNA [DNase I treatment]. In many cases, total RNA prepared using spin-columns or acid guanidium-phenol- chloroform (AGPC) extraction methods contains small amount of genomic DNA. Any contaminating genomic DNA will be amplified along with cDNA, especially when primer pairs are designed within the same exon or from pseudogenes. Amplification from genomic DNA can result in qualitative and quantitative inaccuracies. The genomic DNA degradation and revers transcription steps can be achieved sequentially without purification or heat inactivation of DNase I.