RNA-direct SYBR Green Realtime PCR Master Mix

Intercalation assay with SYBR® Green I

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This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8 (1). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. RNA-direct™ Realtime PCR Master Mix is applicable for TaqMan® assay or hybridization probe assay, in combination with each probe. RNA-direct™ SYBR Green™ Realtime PCR Master Mixcan be applied to an intercalation assay with SYBR® Green I.