GenNext RamDA-seq Single Cell Kit

cDNA synthesis from trace RNA
Full length cDNA can be analyzed
Various RNAs can be detected, and the number of genes detectable is higher than that with conventional technology
Identification of isoforms and alternative splicing
Detection of poly(A) RNA and non poly(A) RNA (histone RNA and lncRNA)
Detection of nuclear RNA (pre mRNA and lncRNA)

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Transcription with Random Displacement Amplification) method for preparing cDNA for NGS analysis from single-cell and Ultra-low amount RNA.RT-RamDA™ is a novel cDNA amplification method utilizing the strand displacement activity of reverse-tr​anscriptas​e. GenNext RamDA-seq™ Single Cell Kit can detect not only poly(A) RNA but also non-poly(A) with high sensitively by using random primer or NSR primer*, allowing the detection of many genes compared with the conventional method.* RamDA-seq™ and RT-RamDA™ are a trademark of RIKEN, Institute of Physical and Chemical Research* NSR primer is abbreviation for Not so random primer.NSR primer is designed random primer to avoid synthesizing cDNA from the rRNAs by removing 6-mers that exactly match the rRNA sequences from N6 random primers.In order to perform more efficient NGS analysis, it is necessary to reduce the rRNA contamination rate.As a countermeasure, we also offer Not so random primers for humans and mice sold separately.