KOD-Plus- Mutagenesis Kit

Site-directed mutagenesis
Substitution (e.g. Point mutation, Point mutation library)
Deletion (e.g. 1 bp – several kbp deletion)
Insertion (e.g. Introduction of His-tag sequence)

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This kit is an inverse PCR (iPCR)-based site-directed mutagenesis kit using KOD DNA polymerase (1)( 2) as a high-fidelity PCR enzyme. This reagent was developed based on a high efficient and efficient PCR reagent, "KOD-Plus- , which consists of KOD DNA polymerase and anti-KOD DNA polymerase antibodies(3) for Hot Start PCR.This kit enables not only the introduction of point mutations, but also the introduction of large insertions and deletions. The PCR fidelity of KOD-Plus- is greater than Taq DNA polymerase (ca. 80-fold); therefore, unexpected, 2nd-site mutations can be reduced. PCR reactions can be performed using standard PCR primers and do not require phosphorylated primers, because this protocol contains a 'Phosphorylation Step' of PCR products.