LEF1 Reporter, BioAssay™ Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)

Kits and Assays / Kits-Molecular Biology, BioAssay™

-20°C

The Wnt/b-catenin signaling pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of b-catenin. b-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription and expression of Wnt-responsive genes.

Intended Use:
Designed for monitoring the activity of Wnt/b-catenin signaling pathway in the cultured cells.

Test Principle:
The kit contains transfection- ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non- inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.

Kit Components:
Reporter (Component A): TCF/LEF luciferase reporter vector + constitutively expressing Renilla luciferase vector. 1x500ul (60ng DNA/ul)
Negative Contol Reporter (Component B): Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector. 1x500ul (60ng DNA/ul)

Storage and Stability:
Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.