BGT, T4 phage, Recombinant, His-Tag (DNA beta-glucosyltransferase, Beta-GT, beta-glucosyltransferase)

Dry Ice

41, 6

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM imidazole, 20% glycerol.

Catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. Is involved in a DNA modification process to protect the phage genome against its own nucleases and the host restriction endonuclease system.

Recombinant corresponding to aa1-351 from human BGT, T4 phage, fused to His-tag at C-terminal, expressed in E. coli.

Molecular Weight:
~41.6kD

Applications:
Suitable for use in the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.