POLR2J, Recombinant, Human, aa1-117, His-Tag (DNA-directed RNA Polymerase II Subunit RPB11-a, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m)

Blue Ice

15, 7

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 5mM DTT, and 2mM EDTA, 40% glycerol.

POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.

Source:
Recombinant protein corresponding to aa1-117 from human POLR2J at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~15.7kD (140aa)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGS EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE MNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.